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  AbstractSolvated L-arginine vasopressin (AVP) and its immobilized RNA aptamer (spiegelmer) were allowed to achieve equilibrium binding in a microchip at a series of selected temperatures. Un yeezys bound AVP were collected and analyzed with matrix-assisted laser desorption鈭昳onization mass spectrometry (MALDI-MS), yielding melting curves that reveal highly temperature-dependent zones in which affinity binding (36-45鈥壜癈) or dissociation (25-33鈥壜癈 and 50-65鈥壜癈) occurs. Additionally, temperature-dependent binding isotherms were  new balance 480 constructed; from these, thermodynamic quantities involved in binding were extracted. The results illustrated a strong change in heat capacity of interaction for this system, suggesting a considerable thermodynamic influence controlling vasop nike dunk ressin-spiegelmer interaction.The article is published online in Biomicrofluidics and is free to access.  WASHINGTON:A 6.1-magnitude earthquake has struck near the coast of Ecuador, just days after the South American country was devastated by a deadly 7.8-magnitude quake, US experts said Wednesday.238 dead as quake devastates Ecuador鈥檚 coast zoneThe epicenter of  stanley shop the quake, which boots kaufen  struck at 8:33 GMT at a depth of 15.7 kilometers (9.8 miles),  new balance 480 was located 25 kilometers west of Muisne and 73 kilometers west-southwest of Propicia, the US Geological Survey said.No tsunami warning has been issued.The USGS indicated on its website that shaking was weak to light in the country's capital Quito and its biggest city Guayaquil.Late Saturday, a 7.8-magnitude quake struck Ecuador's Pacific coast in a zone popular with tourists. The death toll from that quake currently stands at 480 but was set to rise sharply as after authorities warned that 1,700 people were still missing.Anger erupts as 1,700 still missing in Ecuador quakeIn an attempt to rescue survivors potentially trapped under the rubble, sniffer do

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